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cf568 mix n stain kit  (Biotium)


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    Biotium cf568 mix n stain kit
    Immunofluorescence staining of RPMI 8226 trRpn13-MM2 cells Z-slices from a confocal microscopy image of a sample prepared following the described method showing RPMI 8226 trRpn13-MM2 cells undergoing mitosis (left) and at interphase (right) with fluorescence staining for F-actin (phalloidin-ATTO643, red), β-tubulin <t>(TUBB4A-CF568,</t> yellow) and DNA (DAPI, blue). Membrane blebbing is indicated by a white arrow. Scale bar is 2 μm.
    Cf568 Mix N Stain Kit, supplied by Biotium, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotium+cf568+mix+n+stain+kit/https%3A%2F%2Fbiotium%2Ecom%2Fproduct%2Fsynaptoredtmc2m-also-known-as-fm5-95-a-trademark-of-molecular-probes-inc/pmc11078693-139-34-33
    Average 92 stars, based on 2 article reviews
    cf568 mix n stain kit - by Bioz Stars, 2026-09
    92/100 stars

    Images

    1) Product Images from "Protocol for cytoskeleton staining of the semi-adherent multiple myeloma cell line RPMI 8226 by immunofluorescence"

    Article Title: Protocol for cytoskeleton staining of the semi-adherent multiple myeloma cell line RPMI 8226 by immunofluorescence

    Journal: STAR Protocols

    doi: 10.1016/j.xpro.2024.103060

    Immunofluorescence staining of RPMI 8226 trRpn13-MM2 cells Z-slices from a confocal microscopy image of a sample prepared following the described method showing RPMI 8226 trRpn13-MM2 cells undergoing mitosis (left) and at interphase (right) with fluorescence staining for F-actin (phalloidin-ATTO643, red), β-tubulin (TUBB4A-CF568, yellow) and DNA (DAPI, blue). Membrane blebbing is indicated by a white arrow. Scale bar is 2 μm.
    Figure Legend Snippet: Immunofluorescence staining of RPMI 8226 trRpn13-MM2 cells Z-slices from a confocal microscopy image of a sample prepared following the described method showing RPMI 8226 trRpn13-MM2 cells undergoing mitosis (left) and at interphase (right) with fluorescence staining for F-actin (phalloidin-ATTO643, red), β-tubulin (TUBB4A-CF568, yellow) and DNA (DAPI, blue). Membrane blebbing is indicated by a white arrow. Scale bar is 2 μm.

    Techniques Used: Immunofluorescence, Staining, Confocal Microscopy, Fluorescence, Membrane

    Related Articles

    Concentration Assay:

    Article Title: Protocol for cytoskeleton staining of the semi-adherent multiple myeloma cell line RPMI 8226 by immunofluorescence.
    Article Snippet: Approach for minimal distortion of cell morphology by reducing centrifugation Preservation of fine cellular details of semi-adherent or suspension cells for imaging by immunofluorescence is challenging.. This protocol enables staining of floating cells with minimal morphological distortions, as we demonstrate with the semi-adherent multiple myeloma cell line RPMI 8226.. We describe steps to better preserve structural details by fixing, permeabilizing, and staining cells in solution, while minimizing the number of centrifugation steps and centrifugation g-force.

    Article Title: Protocol for cytoskeleton staining of the semi-adherent multiple myeloma cell line RPMI 8226 by immunofluorescence
    Article Snippet: .. We immunostained the RPMI 8226 cells with TUBB4A antibodies at a final concentration of 10 μg /mL from 1:30 dilution of a 300 μg /mL stock concentration obtained following the probe conjugation with Biotium CF568 Mix-n-Stain kit according to the manufacturer’s instructions: https://biotium.com/wp-content/uploads/2017/10/PI-Mix-n-Stain-Antibody-Labeling-Kits.pdf . c. Cover the Eppendorf tubes with aluminum foil to prevent light exposure and incubate them horizontally ∼15 h on a rocking platform (VWR Model 100 at the setting 2) at 4°C. ..

    Conjugation Assay:

    Article Title: Protocol for cytoskeleton staining of the semi-adherent multiple myeloma cell line RPMI 8226 by immunofluorescence.
    Article Snippet: Approach for minimal distortion of cell morphology by reducing centrifugation Preservation of fine cellular details of semi-adherent or suspension cells for imaging by immunofluorescence is challenging.. This protocol enables staining of floating cells with minimal morphological distortions, as we demonstrate with the semi-adherent multiple myeloma cell line RPMI 8226.. We describe steps to better preserve structural details by fixing, permeabilizing, and staining cells in solution, while minimizing the number of centrifugation steps and centrifugation g-force.

    Article Title: Protocol for cytoskeleton staining of the semi-adherent multiple myeloma cell line RPMI 8226 by immunofluorescence
    Article Snippet: .. We immunostained the RPMI 8226 cells with TUBB4A antibodies at a final concentration of 10 μg /mL from 1:30 dilution of a 300 μg /mL stock concentration obtained following the probe conjugation with Biotium CF568 Mix-n-Stain kit according to the manufacturer’s instructions: https://biotium.com/wp-content/uploads/2017/10/PI-Mix-n-Stain-Antibody-Labeling-Kits.pdf . c. Cover the Eppendorf tubes with aluminum foil to prevent light exposure and incubate them horizontally ∼15 h on a rocking platform (VWR Model 100 at the setting 2) at 4°C. ..



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    Immunofluorescence staining of RPMI 8226 trRpn13-MM2 cells Z-slices from a confocal microscopy image of a sample prepared following the described method showing RPMI 8226 trRpn13-MM2 cells undergoing mitosis (left) and at interphase (right) with fluorescence staining for F-actin (phalloidin-ATTO643, red), β-tubulin <t>(TUBB4A-CF568,</t> yellow) and DNA (DAPI, blue). Membrane blebbing is indicated by a white arrow. Scale bar is 2 μm.
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    https://www.bioz.com/product/biotium+cf568+mix+n+stain+kit/https%3A%2F%2Fbiotium%2Ecom%2Fproduct%2Fsynaptoredtmc2m-also-known-as-fm5-95-a-trademark-of-molecular-probes-inc/pmc11078693-139-34-33
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    biotium cf568 mix n stain kit  (Biotium)
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    Immunofluorescence staining of RPMI 8226 trRpn13-MM2 cells Z-slices from a confocal microscopy image of a sample prepared following the described method showing RPMI 8226 trRpn13-MM2 cells undergoing mitosis (left) and at interphase (right) with fluorescence staining for F-actin (phalloidin-ATTO643, red), β-tubulin <t>(TUBB4A-CF568,</t> yellow) and DNA (DAPI, blue). Membrane blebbing is indicated by a white arrow. Scale bar is 2 μm.
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    Immunofluorescence staining of RPMI 8226 trRpn13-MM2 cells Z-slices from a confocal microscopy image of a sample prepared following the described method showing RPMI 8226 trRpn13-MM2 cells undergoing mitosis (left) and at interphase (right) with fluorescence staining for F-actin (phalloidin-ATTO643, red), β-tubulin <t>(TUBB4A-CF568,</t> yellow) and DNA (DAPI, blue). Membrane blebbing is indicated by a white arrow. Scale bar is 2 μm.
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    Image Search Results


    Immunofluorescence staining of RPMI 8226 trRpn13-MM2 cells Z-slices from a confocal microscopy image of a sample prepared following the described method showing RPMI 8226 trRpn13-MM2 cells undergoing mitosis (left) and at interphase (right) with fluorescence staining for F-actin (phalloidin-ATTO643, red), β-tubulin (TUBB4A-CF568, yellow) and DNA (DAPI, blue). Membrane blebbing is indicated by a white arrow. Scale bar is 2 μm.

    Journal: STAR Protocols

    Article Title: Protocol for cytoskeleton staining of the semi-adherent multiple myeloma cell line RPMI 8226 by immunofluorescence

    doi: 10.1016/j.xpro.2024.103060

    Figure Lengend Snippet: Immunofluorescence staining of RPMI 8226 trRpn13-MM2 cells Z-slices from a confocal microscopy image of a sample prepared following the described method showing RPMI 8226 trRpn13-MM2 cells undergoing mitosis (left) and at interphase (right) with fluorescence staining for F-actin (phalloidin-ATTO643, red), β-tubulin (TUBB4A-CF568, yellow) and DNA (DAPI, blue). Membrane blebbing is indicated by a white arrow. Scale bar is 2 μm.

    Article Snippet: We immunostained the RPMI 8226 cells with TUBB4A antibodies at a final concentration of 10 μg /mL from 1:30 dilution of a 300 μg /mL stock concentration obtained following the probe conjugation with Biotium CF568 Mix-n-Stain kit according to the manufacturer’s instructions: https://biotium.com/wp-content/uploads/2017/10/PI-Mix-n-Stain-Antibody-Labeling-Kits.pdf . c. Cover the Eppendorf tubes with aluminum foil to prevent light exposure and incubate them horizontally ∼15 h on a rocking platform (VWR Model 100 at the setting 2) at 4°C.

    Techniques: Immunofluorescence, Staining, Confocal Microscopy, Fluorescence, Membrane